Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly.
نویسندگان
چکیده
In addition to its role as an information carrier in gene expression, RNA is now recognized to carry out a broad range of biological functions, and novel activities continue to emerge on a regular basis. RNA molecules can act as catalysts in reversible phosphodiester-cleavage reactions (Fedor, 2000; Hutchins et al., 1986; Shih and Been, 2002), mediate splicing of premessenger RNA (Collins and Guthrie, 2000; Valadkhan, 2007), silence specific genes (Zamore and Haley, 2005), and act as molecular switches to sense metabolites and regulate the translation of genes into proteins (Barrick and Breaker, 2007; Winkler and Breaker, 2005), to name just a few
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ورودعنوان ژورنال:
- Methods in enzymology
دوره 450 شماره
صفحات -
تاریخ انتشار 2008